The expressed sequence tag of eukaryotic translation initiation factor 5 (
eIF5) from the
Schistosoma japonicum
adult worm cDNA library through subtractive hybridization between male and female worms was analyzed by the bioinformatics method. The overlapping sequences were assembled into one that includes the complete open reading frame (GenBank accession number: AY686501). The full-length cDNA of
SjeIF5 was cloned into a pET-28c
(+) vector, which generated a prokaryotic expression plasmid, and a fusion protein of 18 kDa was induced in
Escherichia coli
. The recombinant expression of
eIF5 protein of
Schistosoma japonicum was purified. The immunoprotection test against schistosomiasis demonstrated that the recombinant protein worked to a certain extent, especially in the reduction of eggs in the liver of the host.
