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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 26, No. 5, 2005, pp. 513-517
Bioline Code: zr05046
Full paper language: Chinese
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 26, No. 5, 2005, pp. 513-517

 en Cloning and Functional Analysis of the Full Length cDNA Sequence of Eukaryotic Translation Initiation Factor 5 in Schistosoma japonicum check for this species in other resources
CHENG Guo-feng, LIN Jiao-jiao, SUN Jun, LI Hao, ZHU Chuan-gang, ZHOU Yuan-cong, CAI You-min

Abstract

The expressed sequence tag of eukaryotic translation initiation factor 5 (eIF5) from the Schistosoma japonicum check for this species in other resources adult worm cDNA library through subtractive hybridization between male and female worms was analyzed by the bioinformatics method. The overlapping sequences were assembled into one that includes the complete open reading frame (GenBank accession number: AY686501). The full-length cDNA of SjeIF5 was cloned into a pET-28c(+) vector, which generated a prokaryotic expression plasmid, and a fusion protein of 18 kDa was induced in Escherichia coli check for this species in other resources . The recombinant expression of eIF5 protein of Schistosoma japonicum was purified. The immunoprotection test against schistosomiasis demonstrated that the recombinant protein worked to a certain extent, especially in the reduction of eggs in the liver of the host.

Keywords
Schistosoma japonicum, eIF5, Expression, Vaccine

 
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