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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 26, No. 6, 2005, pp. 622-626
Bioline Code: zr05061
Full paper language: Chinese
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 26, No. 6, 2005, pp. 622-626

 en Purification and Trypsin Inhibitory Activity of Xenopus laevis check for this species in other resources Serum Albumin
ZHANG Ying-xia, LEE Wen-hui, ZHANG Yun

Abstract

Frog albumin (XpA-serum) was purified from serum of Xenopus laevis check for this species in other resources by a combination of gel filtration and ion exchange chromatography steps. Like Bombina maxima check for this species in other resources albumin, XpA-serum exhibited trypsin inhibitory activity, which was lower than that of B. maxima albumin. XpA-serum at the concentration of 180 nM inhibited 84% activity of trypsin (30 nM). The equilibrium dissociation constants (KD) is 1.44×10-6 M as determined by Surface Plasmon Resonance. Western blot analysis revealed that XpA-serum was also distributed in the skin. It is deduced that serum albumin of amphibian possessing trypsin inhibitory activity can function directly or indirectly as a defensive substance against predators.

Keywords
Amphibian, Xenopus laevis, Serum albumin, Trypsin inhibitor

 
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