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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 26, No. 6, 2005, pp. 652-656
Bioline Code: zr05066
Full paper language: Chinese
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 26, No. 6, 2005, pp. 652-656

 en Isolation of CA/GT Microsatellites from the Paralichthys olivaceus check for this species in other resources Genome
CHANG Yu-mei, SUN Xiao-wen, LI Shao-wu, ZHAO Ying-ying, ZHU Xiao-chen, LIU Hai-jing

Abstract

A library rich in CA/GT microsatellites was constructed from the Paralichthys olivaceus check for this species in other resources genome by combining biotin capture method and radioactive labeling hybridization. Five hundred and twenty six positive clones were obtained through twice screens. Sequencing confirmed 133 microsatellite loci (number of repeats≥5) in 119 positive clones. Of these microsatellites, two (1.5%) had compound repeat motifs, 63 (47.37%) had perfect motifs and 68 (51.13%) had imperfect motifs. Primer pairs were designed in the flanking regions of 22 microsatelites and subjected to PCR amplification. In 8 artificial gynogenesis families, four pairs failed to amplification, one pair was monomorphic, and the rest were polymorphic with an average of 5.2 alleles per locus. Heterozygosities ranged between 0.375 and 0.846, PIC ranged between 0.305 and 0.823. The results suggested that most of the microsatellites we isolated were qualified to be applied to the population genetic studies of P. olivaceus.

Keywords
Paralichthys olivaceus, Microsatellite, Biotin capture, Radioactive labeling

 
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