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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 27, No. 3, 2006, pp. 317-324
Bioline Code: zr06045
Full paper language: Chinese
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 27, No. 3, 2006, pp. 317-324

 en Construction of a Genetic Linkage Map in Fenneropenaeus chinensis check for this species in other resources Using RAPD and SSR Markers
SUN Zhao-ning, LIU Ping, LI Jian, MENG Xian-hong, ZHANG Xiu-mei

Abstract

The primary genetic linkage maps of Fenneropenaeus chinensis check for this species in other resources were constructed by using a "two-way pseudo-testcross" strategy with RAPD and SSR markers. Parents and F1 progeny were used as segregating populations. Sixty-one RAPD primers and 20 pairs of SSR primers were screened from 460 RAPD primers and 44 pairs of SSR primers. These primers were used to analyze the parents and 82 progeny of the mapping family. One hundred and forty-six marker primers (128 RAPDs, 18 microsatellites) were used in the female and 127 primers (109 RAPDs, 18 microsatellites) in the male were used as segregating markers. The female linkage map included eight linkage groups, nine triplets and 14 doublets, spanning 1 173 cM with the average marker density of 11.28 cM. The observed coverage was 59.36%. The male linkage map included 10 linkage groups, 12 triplets and seven doublets, spanning 1 144.6 cM with the average marker density of 12.05 cM. The observed coverage was 62.01%. The construction of the F. chinensis genetic linkage maps here open a new prospect for marker-assisted selection programs, comparative genomics and quantitative trait loci (QTL) gene location and cloning.

Keywords
Fenneropenaeus chinensis; "Yellow Sea 1"; RAPD; SSR; Genetic linkage map

 
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