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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 27, No. 3, 2006, pp. 325-330
Bioline Code: zr06046
Full paper language: Chinese
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 27, No. 3, 2006, pp. 325-330

 en Construction of a cDNA Library of the Prefrontal Cortex of Rhesus Monkey
LI Yi, HUANG Wei, ZHANG Xin, SU Bing

Abstract

Total RNA was extracted from the prefrontal cortex (PFC) of rhesus monkeys with TRIzol reagent. mRNA was further purified from total RNA using a purifying kit for mRNA. According to the manual of cDNA Synthesis Kit (200401), ZAP-cDNA Synthesis Kit (200400),and ZAP-cDNA Gigapack III Gold Cloning Kit (200450) from Stratagene, we constructed a cDNA-library of the PFC of rhesus monkeys. Our aim was to identify brain-function (such as cognition and memory) related genes and gene families. The cDNA library has a capacity of 2.0×106 clones, and 97.3% of them are recombinant clones. The average size of the inserted cDNA fragments is 1.0 kb with the majority of inserts larger than 0.5 kb, which meets the requirement of a standard cDNA library. The PFC cDNA library constructed will be useful in cloning and sequencing brain-function related genes, and in dissecting the alternative splicing patterns of genes expressed in the brain. Meanwhile, it will also serve as a useful resource in understanding the expression regulations within the brain.

Keywords
Rhesus monkey; Prefrontal cortex in rhesus brain; cDNA-library; Genes expressed in brain

 
© Copyright 2006 Kunming Institute of Zoology, the Chinese Academy of Sciences
Alternative site location: http://www.zoores.ac.cn/

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