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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 28, No. 1, 2007, pp. 17-23
Bioline Code: zr07003
Full paper language: Chinese
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 28, No. 1, 2007, pp. 17-23

 en Cloning and Analysis of Fhx/P25 Gene Promoter of Bombyx mori check for this species in other resources Fibroin Protein
LIU Wei-bin, GONG Cheng-liang, XUE Ren-yu, ZHOU Wen-lin, ZHU Xu-xian, CAO Guang-li

Abstract

In order to better understand the model of the molecular mechanisms governing spatially and temporally programmed transcription, the Fhx/P25 gene promoter of the fibroin protein from Bombyx mori check for this species in other resources was cloned and sequenced. An expression vector named pSK-P25-DsRed-PolyA was constructed, in which the reporter gene DsRed was driven by Fhx/P25 promoter. The promoter's activity was then characterized by transient expression assays in BmN cells of B. mori. The results of a sequence analysis showed that the Fhx/P25 promoter possesses the characteristics both of a eukaryotic promoter and a silk gland-specific expression promoter. A conserved TATA box sequence was located at position -28--32 and had the sequence TATAA. There were three CAAT motifs, of which the two CAAT motifs located at positions -110--117 and -90--87 may be active. Secondary structure analysis indicated that the sequence of the promoter region forms a complicated stem?loop structure. This may relate to the tissue speciality or the timing and activity of the protein expression. The transient expression product of the red fluorescent gene, driven by the promoter Fhx/P25, can be observed in cultured BmN cells of B. mori.

Keywords
Bombyx mori; Fhx/P25 gene; Promoter; Sequence analysis

 
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