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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 28, No. 1, 2007, pp. 81-87
Bioline Code: zr07014
Full paper language: Chinese
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 28, No. 1, 2007, pp. 81-87

 en The Effects of Different Cryoprotectants on the Cryo preservation of Rabbit Embryonic Stem Cells
YUAN Xiao-hua, WANG Shu-fen, YANG Shi-hua, LI Ya-hui, CHEN Hong-wei, JI Wei-zhi


An effective freezing-thawing technique is crucial for the clinical application of newly derived rabbit embryonic stem (ES) cells. The aim of this study was try to find an optimal cryopreservation protocol for rabbit embryonic stem cells using slow freezing-rapid thawing without a programmable freezer. We tested the effects of the following cryoprotective agents (CPAs) on the post-thaw survival, proliferation and differentiation capacity of rabbit embryonic stem cells: ethylene glycol (EG), dimethyl sulphoxide (Me2SO, DMSO), trehalose and glutamine. Trypan blue exclusion tests showed that, among the CPA treatments in this study, EG was more toxic to rabbit embryonic stem cells than DMSO. The highest survival rate (83.7%) was obtained when the rabbit embryonic stem cells was cryopreserved with 10%DMSO and 20 mmol/L glutamine in the ES cell culture media. Thawed ES cells kept their pluripotency and differentiation potential.

Rabbit embryonic stem cells; Slow-rate cooling; Cryoprotectants; Glutamine

© Copyright 2007 Kunming Institute of Zoology, the Chinese Academy of Sciences
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