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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 28, No. 2, 2007, pp. 119-125
Bioline Code: zr07021
Full paper language: English
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 28, No. 2, 2007, pp. 119-125

 en Isolation of Microsatellite DNA and Preliminary Genomic Analysis of Mud Carp ( Cirrhina molitorella check for this species in other resources )
CHENG, Fei; YE, Wei & YE, Fu-liang

Abstract

In order to determine the applicability of microsatellite primers developed from common carp ( Cyprinuscario check for this species in other resources ) for genomic analysis in mud carp ( Cirrhina olitorella check for this species in other resources ), 24 primer pairs from common carp were designed to amplify microsatellite loci in the mud carp containing CA, GA, AT and GGGA sequences. Thirteen primers (54%) successfully amplified specific products in the mud carp and 11 primers (48%) showed high polymorphism in the mud carp population. The results indicated that the average number of alleles per locus in the mud carp stocks was 5.2. Average heterozygosity (Ho), unbiased expected heterozygosity (Ho) and polymorphism information content (PIC) in the wild population were 0.61±0.2, 0.8±0.09 and 0.72±0.1 respectively. Several Hardy-Weinberg departure value were significantly departed from Hardy-Weinberg equilibrium. The study showed that microsatellite primers from a species of Cyprinidae can be used for mud carp genetic analysis without much cost or time input.

Keywords
Mud carp (Cirrhina molitorella), Microsatellite, Screening, Genomic polymorphism

 
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Alternative site location: http://www.zoores.ac.cn/

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