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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 28, No. 3, 2007, pp. 303-310
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Bioline Code: zr07047
Full paper language: Chinese
Document type: Research Article
Document available free of charge
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Zoological Research, Vol. 28, No. 3, 2007, pp. 303-310
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Expression of c-fos, Oestradiol and Oestrogen Receptor in the Testis of Rana chensinensis at Different Stages
YANG, Chun & ZHANG, Yu-hui
Abstract
Localisation of FOS, 17β-oestradiol (E2) and oestrogen receptors (ER) was investigated in testes at different stages of the spermatogenetic cycle of Rana chensinensis, using immunocytochemistry. The results showed that E2 and ER are expressed in spermatogonia, spermatocytes, spermatids, spermatozoa, Sertoli cells and Leydig cells from stage I to stage V of spermatogenesis. A strong positive co-expression of E2 and ER was observed in spermatids in stage II and stage III. The staining intensity of E2 and ER in stage III spermatozoa was significantly higher than in stages IV and V (P<0.01). Expression of E2 and ER in Sertoli cells appears in stage I and decreases in intensity from stage II to stage IV, but increases in stage V. Expression of E2 and ER in Leydig cells was higher in stage III than in other stages. FOS was localised in spermatogonia, spermatocytes, spermatids, Sertoli cells and Leydig cells but not spermatozoa. Expression of FOS showed stage-specific characteristics.
Keywords
Rana chensinensis Testis Proto-oncogene Oestradiol-17β Oestrogen receptor Immunocytochemistry
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© Copyright 2007 Kunming Institute of Zoology, the Chinese Academy of Sciences Alternative site location: http://www.zoores.ac.cn/
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