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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 28, No. 4, 2007, pp. 437-442
Bioline Code: zr07066
Full paper language: Chinese
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 28, No. 4, 2007, pp. 437-442

 en Non-monophyly of Rhacophorus rhodopus check for this species in other resources , Theloderma and Philautus albopunctatus check for this species in other resources Inferred from Mitochondrial 16S rRNA Gene Sequences
YU, Guo-hua; RAO, Ding-qi; YANG, Jun-xing & ZHANG, Ming-wang

Abstract

Mitochondrial gene fragments of 16S rRNA gene of four species (Rhacophorus rhodopus, R. reinwardtii, Philautus albopunctatus and P. rhododiscus) from 11 populations were sequenced in this study. Homologous sequences of R. bipunctatus, Theloderma asperum, T. corticale and Buergeria japonica check for this species in other resources were obtained by screening the GenBank database. After excluding all gaps and ambiguous positions, aligned sequences were 500 bp in length with 115 variable sites and 92 parsimony-informative sites. Using B. japonica as an outgroup, phylogenetic relationships were analyzed using Bayesian inference, maximum parsimony and maximum likelihood methods. Our results indicated that neither of R. rhodopus and P. albopunctatus were monophyletic at the species level. The population of R. rhodopus from Hainan Island was more close to R. bipunctatus than to populations of R. rhodopus from Yunnan Province. Furthermore, the populations of R. rhodopus from Yunnan Province can be divided into two main lineages. Theloderma corticale check for this species in other resources and P. rhododiscus were clustered together and T. asperum was nested in P. albopunctatus. We considered that P. albopunctatus Liu and Hu, 1962, was the synonymy of T. asperum Boulenger, 1886, and suggested removing P. rhododiscus from Philautus into the genus Theloderma.

Keywords
Rhacophorus rhodopus, Phylogenetic relationships, 16S rRNA

 
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