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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 28, No. 5, 2007, pp. 465-469
Bioline Code: zr07071
Full paper language: Chinese
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 28, No. 5, 2007, pp. 465-469

 en Characterization of CaPKR-like Expression in Tissues from Crucian and Grass Carp
PENG, Wu; TANG, Ya-nan & HU, Cheng-yu

Abstract

Double-stranded RNA activated protein kinase (PKR) is an important antiviral protein induced by interferon (IFN). PKR was discovered as the enzyme responsible for inhibition of protein synthesis. CaPKR-like is the first fish PKR-like gene isolated from crucian carp. In the present study, the expression of CaPKR-like in the tissues of crucian and grass carp were analyzed by Western blots. In both species, CaPKR-like had a very low level of constitutive expression in un-stimulated tissues, but was up-regulated in all tissues tested in animals stimulated with Poly I:C for seven days. The expression analysis revealed the same characterization of CaPKR-like as mammalian PKR genes.

Keywords
PKR; CaPKR-like; Expression; Crucian carp; Grass carp

 
© Copyright 2007 Kunming Institute of Zoology, the Chinese Academy of Sciences
Alternative site location: http://www.zoores.ac.cn/

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