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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 28, No. 6, 2007, pp. 580-588
Bioline Code: zr07088
Full paper language: Chinese
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 28, No. 6, 2007, pp. 580-588

 en Complete Mitochondrial Genome and Secondary Structures of lrRNA and srRNA of Atractomorpha sinensis check for this species in other resources (Orthoptera, Pyrgomorphidae)
DING, Fang-mei; SHI, Hong-wen & HUANG, Yuan

Abstract

The complete sequence of Atractomorpha sinensis check for this species in other resources mitochondrial genome was determined using long PCR and conserved primer walking approaches. The results showed that the entire mitochondrial genome of A. sinensis is 15 558 bp long with A+T content 74.3%. All 37 genes are conserved in the position observed in that of Locusta migratoria check for this species in other resources . Genes are closely assembled one after the other, leaving a total of 64 bp (excluding the A+T rich region) in intergenic spacers, ranging in size from 1 bp to 16 bp. The extremities of some genes overlap with each other by a few nucleotides, of which 15 overlaps range in size from 1 bp to 8 bp, giving a total of 51 overlapping nucleotides. In the 13 protein coding genes (PCGs), six types of start codons were identified, in which TAA and TAG were used in 12 PCGs as stop codons, except ND5 (TA). Most tRNA genes could form typical secondary structures except tRNASer (AGN), which had an absence of the DHU arm. There are six domains, 49 helices and three domains, 33 helices in the secondary structures of lrRNA and srRNA, respectively. In addition, there is an A+T rich region with 778 bp in length containing a polythymidine stretch that may be involved in replication and/or translation initiation.

Keywords
Mitochondrial genome, rRNA secondary structure, Atractomorpha sinensis, Pyrgomorphidae, Orthoptera

 
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Alternative site location: http://www.zoores.ac.cn/

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