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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 28, No. 6, 2007, pp. 654-658
Bioline Code: zr07097
Full paper language: Chinese
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 28, No. 6, 2007, pp. 654-658

 en Mytomycin Made in China Can Be Used to Isolate Embryonic Stem Cells with Ability in Contributing to Germ Lines
Zhu-ying, WEI; Hua, SHAO; Dong-jun, LIU & Shor-gan, BOU

Abstract

Application of drugs made in China on culture of embryonic stem cells could decrease the cost of ES cell research. In our experiment, mytomycin made in China(Zhejiang Hisun Pharmaceutical Co.,Ltd, authorized H33020786)was used to process feeder cells, from which mouse embryonic stem cells have been isolated and cultured in vitro. When the blastocysts were cultured directly on treated feeder cell layer, the inner cell masses were growing. After 4-6 days culture, ICM-derived outgrowths were separated into clumps, and placed on mitomycin C-treated mouse embryonic fibroblasts feeder layer. Every 2-3 days, passage were made ever since. These ES cells have been proven to have a potential for germ-line contribution.

Keywords
Mytomycin made in China Mouse Embryonic fibroblasts(MEF) Mouse embryonic stem cells

 
© Copyright 2007 Kunming Institute of Zoology, the Chinese Academy of Sciences
Alternative site location: http://www.zoores.ac.cn/

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