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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 29, No. 2, 2008, pp. 127-133
Bioline Code: zr08021
Full paper language: Chinese
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 29, No. 2, 2008, pp. 127-133

 en Comparison of Mitochondrial Control Region Sequences Between Chelydridae check for this species in other resources and Platysternidae check for this species in other resources
Yan, Liang; Zhang, Yan; Wang, Ning; Zhang, Li & Nie, Liu-wang

Abstract

The sequences of mitochondrial control region (CR) of Macroclemys temminckii check for this species in other resources , Chelydra serpentina check for this species in other resources and Platysternon megacephalum check for this species in other resources were obtained using PCR and sequencing techniques, with gene-specific primers, based on the CR and its flanking sequences from other species. The CR lengths of the three species were 1089 bp, 1124 bp and 1119 bp respectively, and the base composition of A+T were 68.97%, 69.34% and 69.44% respectively. One fragment of (TA)20 microsatellite was found in M. temminckii; one fragment of (TATAT) 13 direct tandem repeats followed by (TA)15 microsatellites were found in C.serpentina; and one fragment of (AGTATGTTAT)4 direct tandem repeats followed by (GTTGTTATATAACATAT) 13 repeats were found in P. megacephalum. The distribution of mtDNA microsatellites in tortoises was discussed based on the CR sequences of the three species and other six tortoises published in GenBank. The result suggested that all the nine tortoises have microsatellites, and there are obvious differences both at the site and the sequences.

Keywords
Macroclemys temminckii; Chelydra serpentina; Platysternon megacephalum; mtDNA;Control region Microsatellite

 
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