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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 29, No. 4, 2008, pp. 415-420
Bioline Code: zr08063
Full paper language: Chinese
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 29, No. 4, 2008, pp. 415-420

 en A "2+2 days" Fast Protocol for the Generation of Dendritic Cells from Human Blood Monocytes
LU, Xiao-peng; LI, Jian; SUN, Zheng-hua; ZHAO, Na; LIU, Jie & ZHANG, Hua-tang

Abstract

A "2+2 days" fast protocol for the generation of dendritic cells(DC) from high purity human monocytes "in vitro" has been established. During the 2-step differentiation and activation process, we demonstrated that 2 days of culture with GM-CSF and IL-4 were sufficient to generate immature DCs capable of antigen uptake. Similarly the mature DCs were activated with a cocktail of rTNF-α, IL-1β, IL-6, and PGE2 from immature DC in 2 days. This "2+2 days" fast DC had the same phenotype and function as the “6+2” standard DC.

Keywords
Monocyte; Dendritic cell; "2+2 days" fast method

 
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