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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 29, No. 4, 2008, pp. 421-426
Bioline Code: zr08064
Full paper language: Chinese
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 29, No. 4, 2008, pp. 421-426

 en Establishment and Characterization of THP-1 Cell Model Stably Expressing HIV-1 Auxiliary Protein Rev
ZHOU, Hui-fang; LU, Xiao-peng; SUN, Zheng-hua; GUO, Yan & ZHANG, Hua-tang

Abstract

HIV-1 auxiliary proteins play a critical role in both the infection of HIV-1 target cells and the morbidity of AIDS. Rev, as one of the HIV-1 auxiliary proteins, is essential for the replication of HIV-1. As a membrane-shuttle protein, it can regulate the transportation of HIV-1 structural protein’s mRNA. The rev gene from HIV-1 was transfected into THP-1 cells. The Rev-stably-expressed cell model was generated by electroporation and then sorted and selected with G418. The cell model was characterized at both mRNA and protein levels with an RT-PCR assay, fluorescent observation, and flow cytometry analysis. The rev gene was successfully transfected into THP-1 cells and stably expressed. This is a useful result for future research of the interaction between Rev and cells.

Keywords
Rev; THP-1; Stable expression

 
© Copyright 2008 Kunming Institute of Zoology, the Chinese Academy of Sciences
Alternative site location: http://www.zoores.ac.cn/

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