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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 29, No. 6, 2008, pp. 599-602
Bioline Code: zr08091
Full paper language: Chinese
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 29, No. 6, 2008, pp. 599-602

 en Microsatellite Variation Within Three Populations of Inbred C57BL/6J Strain
Niu, Yi-dong & Liang, Shu-long

Abstract

The goal of this study was to investigate the genetic stability of the C57BL/6J (B6) inbred mouse strain maintained in different breeders. Three populations of B6, Pop1 and Pop2 purchased from Beijing and Pop3 purchased from Shanghai, were examined. Fifteen microsatellite loci reported to be polymorphic among inbred strains were amplified using FAM labeled primers and genotyped with ABI Prism 377 automated sequencer. Seven loci were found polymorphic, and all the loci were homozygous in all the three populations. The present study indicates that genetic variation occurs in different B6 populations although they are still inbred in each breeder. The mechanism of genetic variation is not well understood now, but it is very important to know the precise content of the B6 genome before use of this strain in research.

Keywords
C57BL/6J strain; Microsatellite locus; Genetic variation

 
© Copyright 2008 Kunming Institute of Zoology, the Chinese Academy of Sciences
Alternative site location: http://www.zoores.ac.cn/

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