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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 30, No. 1, 2009, pp. 59-64
Bioline Code: zr09011
Full paper language: Chinese
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 30, No. 1, 2009, pp. 59-64

 en Sex Pheromone Components of the Female Black Cutworm Moth in China: Identification and Field Trials
Xiang, Yu-yong; Yang, Mao-fa & Li, Zi-zhong


The sex pheromone blend of a China strain of the black cutworm moth Agrotis ypsilon check for this species in other resources (Rottemberg) (Lepidoptera: Noctuidae) was investigated. Chemical analysis of pheromone gland extracts of 3-day-old females showed that individual isolated glands contained only very small amounts of pheromone. The high-resolution gas chromatography combined with mass spectrometry (GC-MS) analysis showed the presence of Cis-7-dodecenyl acetate (Z7-12:Ac, A), Cis-9-tetradecenyl acetate (Z9-14:Ac, B), Cis-11-hexadecenyl acetate (Z11-16:Ac, C), Cis-8-dodecenyl acetate (Z8-12:Ac, E) and Cis-5-decenyl acetate (Z5-10:Ac, D) in biologically active pheromone gland extracts. An extract of one gland from a day 3 female gave the following values for the gland components: 0.245±0.098 ng for Z7-12:Ac, 0.080±0.031 ng for Z9-14:Ac, 0.089±0.033 ng for Z11-16:Ac, 0.085±0.031 ng for Z5-10:Ac, 0.105±0.065 ng for Z8-12:Ac per female. The percentages of Z7-12:Ac, Z9-14:Ac, Z11-16:Ac, Z5-10:Ac and Z8-12:Ac in pheromone gland extracts from individual females were (mean±SE) 40.451±13.66, 13.176±5.279, 14.943±5.142, 14.392±6.10 and 17.225±9.792 respectively, and the percentages of Z7-12:Ac, Z9-14:Ac and Z11-16:Ac were 58.75±9.429, 18.91±7.539 and 22.34±7.209. Field trials indicated that each single component of sex pheromone was non-effective and captured no males. The lures baited with duality compound of AB (3∶1) had a certain attraction to males, the mean captured number was 2.6. The attraction ability of the lures baited with ternary compound of ABC (3∶1∶1) to males added significantly, the mean captured number per trap was 7.40, which was 2.8 times of duality compound of AB (3∶1). The contents of sex pheromone could obviously affect its capture ability to males, the mean captured number was the highest at the dosage of 200 µg.

Agrotis ypsilon Chemical analysis Field trial Gas chromatography/High-resolution mass spectrometry Internal standard Sex pheromone Standard chemicals

© Copyright 2009 Kunming Institute of Zoology, the Chinese Academy of Sciences
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