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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 30, No. 2, 2009, pp. 131-136
Bioline Code: zr09021
Full paper language: English
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 30, No. 2, 2009, pp. 131-136

 en Chromosome Preparation and Preliminary Observation of Two Amphioxus Species in Xiamen
Zhang, Qiu-jin; Li, Guang; Sun, Yi & Wang, Yi-quan

Abstract

Amphioxus has an important evolutionary position as a result of their phylogenetic position relative to vertebrates. Understanding their chromosomes would provide key points in the study of evolutionary biology and comparative genomics. The difficulty in preparing amphioxus chromosomes currently provides a significant hurdle in this research. In the current study, we describe an improved method for metaphase preparation from amphioxus embryos and methodology for preparing metaphase spreads from regenerative somatic cells. Chromosomes of two amphioxus species from Xiamen waters in China are also observed. The diploid chromosome number was found to be 40 in Branchiostoma belcheri check for this species in other resources , while B. japonicum has 36, confirming the two are distinct species from cytotaxonomic viewpoint.

Keywords
Amphioxus (lancelet); Branchiostoma belcheri; B. japonicum; Chromosome; Regenerative cell

 
© Copyright 2009 Kunming Institute of Zoology, the Chinese Academy of Sciences
Alternative site location: http://www.zoores.ac.cn/

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