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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 30, No. 2, 2009, pp. 165-170
Bioline Code: zr09026
Full paper language: English
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 30, No. 2, 2009, pp. 165-170

 en Isolation and Molecular Cloning of Insulinotropic Peptide from the Skin Secretions of Amolops loloensis check for this species in other resources and the Study of Its Bioactivitie
Feng, Hao; Li, Zong-jie; Ly, Yi; Wang, Mei-juan & Li, Jian-xu


To investigate the bioactive component from the skin secretion of Amolops loloensis check for this species in other resources , isolated peaks with insulin-releasing activity were purified and structurally determined. Skin secretions were isolated by Gel filtration and Reversed phase high-performance liquid chromatography, and tested with insulin-releasing assay. The amino acid sequence of bioactive peak was determined by Edman degradation and identified by gene clone method. A 16-amino peptide with obvious insulin-releasing activity was obtained and sequenced as FMPIVGKSMSGLSGKL-NH2 , which was designated amolopin-1. The precursor peptide composed of 64 amino acid residues deduced from cloned skin cDNA (open reading frame 192 bp) exhibited a highly-conserved signal peptide (22aa), an acidic amino acid residue-rich domain and an amolopin-1 encoding domain. Structural alignment with database records revealed that a novel insulinotropic peptide was obtained from skin secretion of Amolops loloensis therefore further study of its acting mechanism and pharmacokinetics may lead to the discovery of a new treatment for diabetes.

Amolops loloensis Skin secretion Insulinotropic peptide Insulin release

© Copyright 2009 Kunming Institute of Zoology, the Chinese Academy of Sciences
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