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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 30, No. 2, 2009, pp. 171-177
Bioline Code: zr09027
Full paper language: English
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 30, No. 2, 2009, pp. 171-177

 en Effects of the Antimicrobial Peptide OH-CATH on Escherichia coli check for this species in other resources
Chen, Xin-xin; Yu, guo-yu; Zhan, Yan; Zhang, Yun; Shen, Ji-hong & Lee, Wen-hui

Abstract

OH-CATH is a novel cathelicidin identified from king cobra. It showed strong antibacterial activity against various bacteria in the presence of 1% NaCl and no haemolytic activity toward human red blood cells even at a high concentration. OH-CATH might serve as model molecules for the development of antimicrobial drugs. Understanding the action mechanism of OH-CATH and the reason for its selectivity against microbes is very important for this purpose. The bactericidal effect of the king cobra antimicrobial peptide OH-CATH on Gram-negative Escherichia coli (ATCC 25922) is observed by scanning electron microscopy (SEM) and transmitted electron microscopy (TEM). The SEM and TEM results suggested that the bactericidal mechanism of OH-CATH against Escherichia coli check for this species in other resources happened in three steps. Firstly, OH-CATH attached to the negatively charged bacterial wall by positively charged amino acid residues. In the second step, the accumulated OH-CATH aggregated and damaged the bacteria membrane in a pore-forming manner. In the last step, with the damage of cell permeability, the contents of the cells were released and eventually cells died.

Keywords
Antimicrobial peptide OH-CATH Escherichia coli Electron microscopy

 
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