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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 30, No. 3, 2009, pp. 262-266
Bioline Code: zr09041
Full paper language: Chinese
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 30, No. 3, 2009, pp. 262-266

 en Cloning of Sheep tnp2 Gene and Transcription Analysis of Round Spermatid Cells in the in vitro Co-culture System
Bai, Yin-ba-tu; Luo, Fen-hua; Hu, Tian-yuan; Hou, Yue & WuU, Ying-ji

Abstract

The transition protein-2 gene (tnp2) have been reported as the round spermatid-specific marker gene. However, the DNA sequence of tnp2 gene in Ovis aries check for this species in other resources have not been reported. In order to study the sheep round spermatid-specific marker gene, we designed primers according to the reported Bos taurus tnp2 gene cDNA sequences in the GenBank. The partial CDS was amplified by RT-PCR. The PCR fragment was inserted into the T-A cloning vector pMD19-T. The partial nucleotide sequences of Mongolia sheep tnp2 gene were compared with the counterpart sequences of Bos taurus, and the nucleotide homology was 95.3%. Using the primers designed according to the tnp2 cDNA sequence, the tnp2 gene transcription expression in the co-cultured cells derived from four-months old sheep testis was assessed by RT-PCR. The results demonstrate that the round spermatid cells are generated in the co-culture system until ten weeks in vitro. The cDNA cloning and sequencing lay down a foundation for further study on spermatogenesis of sheep.

Keywords
Sheep; tnp2 gene; Cloning; Sequence analysis; Round spermatid

 
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Alternative site location: http://www.zoores.ac.cn/

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