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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 30, No. 6, 2009, pp. 620-626
Bioline Code: zr09094
Full paper language: Chinese
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 30, No. 6, 2009, pp. 620-626

 en Kinship Analysis of One Broodstock Population of Large Yellow Croaker Pseudosciaena crocea check for this species in other resources
Chang, Yu-mei; Xu, Wan-tu; Chi, Bing-jie; Gao, Guo-qiang; Han, Qi-xia; He, Wei; Sun, Xiao-wen & Liang, Li-qun

Abstract

Present broodstocks of large yellow croaker are borne from extremely small numbers of base population. Thus, it is necessary to analyze kinship of broodstocks in order to avoid inbreeding that will bring out the reduction of individual survival and growth. This paper reports kinship reconstruction and genetic diversity in 103 broodstocks of large yellow croaker by utilizing 23 microsatellite markers. Genetic diversities of 103 croakers at 23 loci pronounce that there are 134 alleles in total and an average of 5.82, and the observed average heterozygosity of 0.599 3, demonstrating that these broodstocks still maintain genetic variability to some extent. The results of sibling groups reconstructed are not identical using two methods of Likelihood and 2-allele recombinatorial optimization. However, the evidence of close relationship between broodstocks is confirmed. The mating combinations are compared between these two methods, as a result of 85% identity and a final selection of 2-allele method. This study aims at finding a better way to avoid inbreeding occurred in broodstocks, and facilitating the aquaculture market of large yellow croaker, meanwhile, offering methods and statistical models to artificial propagation in other marine fish species.

Keywords
Large yellow croaker; Microsatellite markers; Kinship; Inbreeding

 
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