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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 30, No. 6, 2009, pp. 639-644
Bioline Code: zr09097
Full paper language: English
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 30, No. 6, 2009, pp. 639-644

 en Physical Blocking Neural Tube Closure Affects Radial Intercalation and Neural Crest Midline-directed Migration in Xenopus check for this species in other resources Dorsal Explants
Shi, Yu; Zhao, Shu-hua & Mao, Bing-yu


Neural tube defects (NTDs) are severe congenital malformation diseases, which occur in 1 out of 1000 births in human. In Xenopus check for this species in other resources , several tissue movements are involved in the neural tube closure process. Immediately after the neural tube fusion, the neural crest cells get monopolar protrusion toward dorsal midline and migrate to form the roof of the neural tube. At the same time, radial intercalation takes place from the ventral neural tube and forces it to be single-layered. Here, we physically block the neural tube closure to test the cell movements and the following patterning in Xenopus laevis check for this species in other resources explants. The results show that the single-layered neural tube fails to form and the neural crest cells remain at the lateral regions in the explants with NTDs. However, the patterning of the neural tube is not affected as indicated by the normal expression of the preneural genes. These results indicate a requirement of the neural tube fusion for the radial intercalation and the dorsal midline directed neural crest migration, but not for the dorsal-ventral patterning of the neural tube.

Xenopus laevis; Neural tube defect; Neural tube closure; Neural crest; Morghogenesis; Intercalation; Dorsoventral patterning

© Copyright 2009 Kunming Institute of Zoology, the Chinese Academy of Sciences
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