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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 31, No. 2, 2010, pp. 113−121
Bioline Code: zr10018
Full paper language: English
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 31, No. 2, 2010, pp. 113−121

 en Characterization of Two Human Lung Adenocarcinoma Cell Lines by Reciprocal Chromosome Painting
Peng, Kun-Jing; Wang, Jin-Huan; Su, Wei-Ting; Wang, Xi-Cai; Yang, Feng-Tang & Nie, Wen-Hui

Abstract

Lung cancer is a leading cause of cancer death worldwide. Some lung cancer patients correlate with a gas of radon besides smoking. To search for common chromosomal aberrations in lung cancer cell lines established from patients induced by different factors, a combined approach of chromosome sorting, forward and reverse chromosome painting was used to characterize karyotypes of two lung adenocarcinoma cell lines: A549 and GLC-82 with the latter line derived from a patient who has suffered long-term exposure to environmental radon gas pollution. The chromosome painting results revealed that complex chromosomal rearrangements occurred in these two lung adenocarcinoma cell lines. Thirteen and twenty-four abnormal chromosomes were identified in A549 and GLC-82 cell lines, respectively. Almost half of abnormal chromosomes in these two cell lines were formed by non-reciprocal translocations, the others were derived from deletions and duplication/or amplification in some chromosomal regions. Furthermore, two apparently common breakpoints, HSA8q24 and 12q14 were found in these two lung cancer cell lines.

Keywords
Lung adenocarcinoma cell lines; Chromosome sorting; Chromosome painting; Cytogenetic characterization

 
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Alternative site location: http://www.zoores.ac.cn/

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