search
for
 About Bioline  All Journals  Testimonials  Membership  News  Donations


Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 31, No. 2, 2010, pp. 141-146
Bioline Code: zr10021
Full paper language: English
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 31, No. 2, 2010, pp. 141-146

 en Selection of Reference Genes in Transcription Analysis of Gene Expression of the Mandarin Fish, Siniperca chuasti check for this species in other resources
Zhou, Rui-Xue; Meng, Tao; Meng, Hai-Bo; Cheng, Dun-Xue; Bin, Shi-Yu; Cheng, Jia; Fu, Gui-Hong; Chu, Wu-Ying & Zhang, Jian-She

Abstract

At present,transcription analysis of gene expression commonly uses housekeeping genes as control for normalization.In this study, the expression levels of three housekeeping genes including GAPDH, β-actin, and 18S rRNA in six tissues and five developmental stages of the Mandarin fish Siniperca chuasti check for this species in other resources were assayed with quantitative real-time PCR (qPCR).Differences in expression levels were analyzed using geNorm program. The results demonstrate that β-actin is the most stable gene at developmental stages and GAPDH is the most stable in different tissues. While 18S rRNA expression during development is differentially regulated, which indicates it is suitable as an internal control for gene expression normalization at the developmental level. Overall, the data suggest that the two most stable housekeeping genes are enough to accurately calibrate gene expression in S. chuatsi. The significance of this study provided convincing references and methodology for housekeeping gene selection and normalization in gene expression analysis with regular PCR or qPCR.

Keywords
Reference genes; geNorm program; Gene expression; Real-time PCR

 
© Copyright 2010 Kunming Institute of Zoology, the Chinese Academy of Sciences
Alternative site location: http://www.zoores.ac.cn/

Home Faq Resources Email Bioline
© Bioline International, 1989 - 2017, Site last up-dated on 16-Oct-2017.
Site created and maintained by the Reference Center on Environmental Information, CRIA, Brazil
System hosted by the Internet Data Center of Rede Nacional de Ensino e Pesquisa, RNP, Brazil