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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 31, No. 2, 2010, pp. 147-153
Bioline Code: zr10022
Full paper language: English
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 31, No. 2, 2010, pp. 147-153

 en RAPD-SCAR Markers for Genetically Improved NEW GIFT Nile Tilapia ( Oreochromis niloticus niloticus check for this species in other resources L.) and Their Application in Strain Identification
Li, Si-Fa; Tang, Shou-Jie & Cai, Wan-Qi

Abstract

Abstract: The NEW GIFT Nile tilapia ( Oreochromis niloticus niloticus check for this species in other resources L.) is a nationally certificated new strain selected over 14 years and 9 generations from the base strain of GIFT Nile tilapia, introduced in 1994. This new variety has been extended in most of areas of China. The management of genetically improved strains, including the genetic markers for identification is needed urgently. RAPD analysis was conducted and their conversion to SCAR markers was developed. From NEW GIFT Nile tilapia, two strain-specific RAPD bands, S304624 bp and S36568 bp were identified. The strain-specific RAPD bands were gel-purified, cloned, and sequenced. Locus-specific primers were then designed to amplify the strain-specific bands. PCR amplification was conducted to test the variations in allele frequencies of two converted SCAR markers among the NEW GIFT Nile tilapia and its base strains, as well as 7 additional farmed strains worldwide. The frequency of SCAR marker I (553 bp) was 85.7% in NEW GIFT Nile tilapia, but 16.7% in the base strain. The frequency of SCAR marker II (558 bp) was 91.4% in NEW GIFT Nile tilapia, but 0% − 70% in the 7 other strains. In order to confirm the utility of these two markers, an examination was conducted for a wild population from Egypt, resulted the frequency of SCAR I and II was 10% and 70%, respectively, much lower than that of New GIFT strain. The increase in allele frequency of these two SCAR markers suggests that these markers might be genetically linked to the quantitative trait loci (QTL) underlining the performance traits by long term selection, and indicate the bright potential of SCAR marker technology for tracking generations during selection progress and for distinguishing among genetically improved strain and other strains.

Keywords
NEW GIFT; Strain; Nile tilapia; RAPD-SCAR marker; Tracking; Identification

 
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