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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 31, No. 3, 2010, pp. 239-249
Bioline Code: zr10032
Full paper language: Chinese
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 31, No. 3, 2010, pp. 239-249

 en Molecular Characterization and Evolutional Analysis of Liportein Lipase (LPL) and Hepatic Lipase (HL) Gene in Chinese sturgeon and Other Six Freshwater Fishes
HUANG, Yan; LIANG, Xu-Fang; WANG, Lin; LI, Guang-Zhao; Liu, Xiu-Xia & YAO, Yu

Abstract

In order to study the structural, functional and molecular evolutional relationship of fish liportein lipase (LPL) and hepatic lipase (HL) genes, seven liver LPL and HL cDNA partial sequences were isolated from Acipenser sinensis, Hypophthalmichthys molitrix, Aristichthys nobilis, Ctenopharyngodon idellus, Cirrhinus molitorella, Oreochromis niloticus, Channa maculate by RT-PCR. Three full-length cDNA sequences of LPL, HL of Acipenser sinensis and LPL of Hypophthalmichthys molitrix were obtained by RACEs. From the sequence analysis and homologous results, the amino acid sequences of LPL and HL are relatively conserved in mammals, birds and fishes. Taken together with these obtained amino acid sequences and sequences of all known LPL, HL, EL and PL from other vertebrates, a phylogenetic tree was constructed by neighbor-joining method. The result supports that all of them belong to lipase family.

Keywords
LPL HL Gene cloning Sequence analysis Molecular evolution Fresh water fishes

 
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