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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 31, No. 4, 2010, pp. 333-344
Bioline Code: zr10045
Full paper language: Chinese
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 31, No. 4, 2010, pp. 333-344

 en Sequencing and Analysis of the Complete Mitochondrial Genome of Pseudopodoces humilis check for this species in other resources (Aves, Paridae)
Yang, Chao; Lei, Fu-Min & Huang, Yuan

Abstract

The complete sequence of Pseudopodoces humilis check for this species in other resources mitochondrial genome was determined by using long PCR and conserved primers walking approaches. The results showed that the entire mitochondrial genome of Pseudopodoces humilis was 16 809 bp in length with 52.9% A+T content, and the 37 genes had the same gene order with that of Gallus gallus. All protein coding genes of the Pseudopodoces humilis mitochondrial genome started with ATG codon, except for COIwith GTG. For terminate codon usage, most of genes terminate with codons TAA or TAG, ND1 and ND5 were AGA, COII was AGG, and the COIII and ND4 genes had an incomplete termination codon (T). The secondary structures of 22 tRNA were predicted, all tRNA can form typical secondary structure, except tRNASer ( AGN ) with an absence of DHU arm, and tRNAPhe with an extra nucleotide inserted in the TψC arm. The predicted secondary structures of 12S rRNA and 16S rRNA exhibit 47 helics in 3 structural domains and 60 helics in 6 domains, respectively. The control region of Pseudopodoces humilis located between tRNAGlu and tRNAPhe with 1 240 bp in length, and has structure domains found in that of the other birds.

Keywords
Pseudopodoces humilis; Passeriformes; Paridae; Pseudopodoces; Complete mitochondrial genome; RNA secondary structure; Central conserved dormain

 
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