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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 31, No. 4, 2010, pp. 361-369
Bioline Code: zr10048
Full paper language: Chinese
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 31, No. 4, 2010, pp. 361-369

 en Population Genetic Differentiation of Phrynocephalus axillaris check for this species in other resources in East of Xinjiang Uygur Autonomous Region Based on Sequence Variation of Mitochondrial ND4-tRNALeu Gene
Li, Jun; Guo, Xian-Guang & Wang, Yue-Zhao

Abstract

A 838 bp fragment of mtDNA ND4-tRNALeu gene was sequenced for 66 individuals from five populations (DB: Dabancheng, TU: Turpan, SS: Shanshan, HL: Liushuquan, HD: East district of Hami) of Phrynocephalus axillaris check for this species in other resources distributed in east of Xinjiang Uygur Autonomous Region. Seventeen haplotypes were identified from 29 nucleotide polymorphic sites in the aligned 838 bp sequence. Excluding DB, there were relatively high haplotype diversity (0.600±0.113) ≤ h ≤ 0(.922±0.012) and low nucleotide diversity (0.00082±0.00020) ≤ π ≤ (0.00917±0.00037) in the whole and the rest four populations. The result of phylogenetic analysis showed that the haplotypes from five populations formed clade?(DB+TU+SS) and clade? (HL+HD). Network analysis further revealed the phylogeographical structure of these five populations. Analysis of molecular variance (AMOVA) indicated that the significant genetic divergence (81.67%, P<0.01) existed among populations, which was not obviously correlated with the geographic distances between populations inferred from isolation-with-distance analysis (r=0.5324, P=0.1196). The demography analysis implied that TU and HL might have experienced the recent population expansion; SS and HD might be relatively stable. With extremely low genetic diversity, DB might be influenced by the special environment and be experiencing the bottleneck effect. It followed that the significant genetic differentiation among the five populations of P. axillaris might be the consequence of habitat changes resulting from the geological events and climate oscillation since Pleistocene and genetic drift.

Keywords
Phrynocephalus axillaris; ND4-tRNA Leu; Population genetic differentiation; East of Xinjiang Uygur Autonomous Region

 
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