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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 31, No. 4, 2010, pp. 387-394
Bioline Code: zr10052
Full paper language: Chinese
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 31, No. 4, 2010, pp. 387-394

 en Identification and Characterization of Myostatin Gene in Rough-skinned Sculp, Trachidermus fasciatus check for this species in other resources
Lu, Xiang-Yun; Zhang, Ying; Wang, Xing-Guo; Zhang, Yan-Ping; Xu, Jian-Rong; Lai, Ren & Gu, Zhi-Liang

Abstract

Myostatin is a member of the TGF-β superfamily and acts as a negative regulator of skeletal muscle growth. The characterization of the myostatin gene and its expression in Trachidermus fasciatus check for this species in other resources was reported in the current study. A full-length of 2 568 bp myostatin cDNA sequence in T. fasciats was cloned by 5' and 3' RACE, which included a 1 128 bp complete ORF encoding a 375 amino acid peptide, a 106 bp long 5'-UTR and a 1134 bp long 3'UTR. As other MSTN, the putative peptide contains a 22 amino acids long signal peptide, a conserved RXXR proteolytic processing site, and 10 conserved cysteine residues in the C terminal of the protein. The Trachidermus fasciatus MSTN has high homology with Umbrina cirrosa, Morone saxatilis, Morone americana, Morone chrysops myostatin while has low homology with mammalian and birds myostatin. The phylogenetic analysis showed that the T. fasciatus myostatin had the closest relationship with U. cirrosa. In the four examined tissues, the myostatin gene was highly expressed in muscle and intestine and weakly expressed in brain and liver. These results suggested that the fish myostatin gene might not only play roles in muscle development but also contribute to other biological functions.

Keywords
Trachidermus fasciatus; Myostatin; RACE (rapid amplify cDNA end); Tissue expressional pattern

 
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