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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 31, No. 4, 2010, pp. 401-407
Bioline Code: zr10054
Full paper language: Chinese
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 31, No. 4, 2010, pp. 401-407

 en Monitoring Inbreeding of WHBE Rabbits Using Microsatellites
Chen, Min-Li; Zhu, Liang; Cai, Yue-Qin; Yu, Jia; Xu, Xiao-Ping; Tu, Jue; Xiao, Hui & Pan, Yong-Ming

Abstract

Microsatellite polymorphisms were analyzed in F5, F6 and F7 WHBE rabbits ( Oryctolagus cuniculus check for this species in other resources ) to monitor inbreeding. Out of 21 microsatellite loci, 11 were successfully amplified and showed polymorphic. For the F5 WHBE rabbits, the number of alleles per locus (Na) ranged from 3 to 9 and the mean effective number of alleles (Ne) was 1.81. The mean value of observed heterozygosity (Ho) and polymorphism information content (PIC) of the 11 polymorphic loci were 0.381 and 0.524, respectively. The power of cumulative discrimination (CDP) was 1.0. The value of cumulative exclusion probability of the 11 polymorphic loci in the absence (CPE-1) or in the presence of genetic information on the first parent (CPE-2) was 0.926 and 0.993, respectively. For the F6 WHBE rabbits, Na per locus ranged from 3 to 8 and the mean value of Ne was 1.68. The mean value of Ho, PIC, CDP, CPE-1, CPE-2 of the 11 polymorphic loci were 0.356, 0.548, 1.0, 0.931, 0.994 respectively. For the F7 WHBE rabbits, Na per locus ranged from 2 to 6 and the mean value of Ne was 1.51. The mean value of Ho, PIC, CDP, CPE-1, CPE-2 of the 11 polymorphic loci were 0.287, 0.498, 1.0, 0.891, 0.986 respectively. The average number of effective alleles and the average observed heterozygosity were decreasing continuously in F 5, F6 and F7, suggesting that the purity of WHBE rabbits was increasing continuously.

Keywords
WHBE rabbit; Inbreeding; Microsatellite; Polymorphism analysis

 
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