Microsatellite polymorphisms were analyzed in F
5, F
6 and F
7 WHBE rabbits (
Oryctolagus cuniculus
) to monitor inbreeding. Out of 21 microsatellite loci, 11 were successfully amplified and showed polymorphic. For the F
5 WHBE rabbits, the number of alleles per locus (Na) ranged from 3 to 9 and the mean effective number of alleles (
Ne) was 1.81. The mean value of observed heterozygosity (Ho) and polymorphism information content (PIC) of the 11 polymorphic loci were 0.381 and 0.524, respectively. The power of cumulative discrimination (
CDP) was 1.0. The value of cumulative exclusion probability of the 11 polymorphic loci in the absence (
CPE-1) or in the presence of genetic information on the first parent (
CPE-2) was 0.926 and 0.993, respectively. For the F
6 WHBE rabbits, Na per locus ranged from 3 to 8 and the mean value of Ne was 1.68. The mean value of
Ho, PIC, CDP, CPE-1, CPE-2 of the 11 polymorphic loci were 0.356, 0.548, 1.0, 0.931, 0.994 respectively. For the F
7 WHBE rabbits, Na per locus ranged from 2 to 6 and the mean value of Ne was 1.51. The mean value of Ho, PIC, CDP, CPE-1, CPE-2 of the 11 polymorphic loci were 0.287, 0.498, 1.0, 0.891, 0.986 respectively. The average number of effective alleles and the average observed heterozygosity were decreasing continuously in F 5, F
6 and F
7, suggesting that the purity of WHBE rabbits was increasing continuously.