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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 31, No. 5, 2010, pp. 469-475
Bioline Code: zr10064
Full paper language: Chinese
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 31, No. 5, 2010, pp. 469-475

 en Identification of zRAP55,a gene preponderantly expressed in Stages I and II oocytes of zebrafish
Cai-Lian, Zhao; Qi-Wen, Yang; Jia-Rui, Hu; Ding, Ye; Wu-Ming, Gong; Hai-Ying, Lu; Zong-Yun, Xu; Xun-Pu, Zhang & Ping, Song

Abstract

In an in silico search for gonand specific expressed genes, we have identified zRAP55 which is enriched in the ovary of zebrafish . zRAP55 encodes a protein of 382 amino acids with a highly conserved Lsm domain. zRAP55 protein shares more than 56% identities with that of other vertebrate species. RT-PCR results show that it is predominantly expressed in the ovary. In situ hybridization and immunohistochemistry studies reveal that zRAP55 is ubiquitously dispersed throughout the cytoplasm of stages I and II oocytes, whereas no expression is observed in stages III and IV oocytes. As an RNA associated protein, zRAP55 might function in the control of protein translation at the early stages of oogenesis in zebrafish.

Keywords
Lsm domain; zRAP55; Oogenesis; Immunohistochemistry; Zebrafish

 
© Copyright 2010 Kunming Institute of Zoology, the Chinese Academy of Sciences
Alternative site location: http://www.zoores.ac.cn/

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