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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 32, No. 3, 2011, pp. 267-276
Bioline Code: zr11038
Full paper language: Chinese
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 32, No. 3, 2011, pp. 267-276

 en Expression analysis of 5 hsp70 genes in Tetrahymena thermophila check for this species in other resources
Feng, Li-Fang; Chang, Yue; Yuan, Dong-Xia & Miao, Wei

Abstract

Thirteen hsp70 genes with complete conserved domains were identified in Tetrahymena thermophila check for this species in other resources , and expression of five similar and non-intron hsp70 genes were analyzed. Under heat shock conditions of 37, 39 and 41°C, hsp70-2 mRNA had the highest relative expression level, suggesting it is closely related to heat shock. The basal level of constitutive T. thermophilahsp70-4 gene was high during 20 physiological/developmental stages of growth, starvation and conjugation, and it changed little upon exposure to heat shock: evidence that hsp70-4 is an hsc70 gene. The hsp70-4 cDNA is 2 208 bp long, and contains an open reading frame of 1 959 bp encoding 635 amino acids. Microarray data indicated that T. thermophilahsp70-3 gene probably participated in early starvation (0-12 h) stress and late conjugation (6-10 h) events, such as new macronuclear and micronuclear anlagen formation and old macronuclear elimination. However, hsp70-5 gene possibly participates in late starvation (12-15 h) stress and early conjugation (0-6 h) events such as micronuclear meiosis, micronuclear exchange and pronuclear fusion. Blast2GO indicated that they participated in dissimilar biological processes, suggesting hsp70-3 and hsp70-5 perform different functions.

Keywords
Tetrahymena thermophila; Real time quantitative PCR; Microarray; RACE; Expression analysis

 
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