search
for
 About Bioline  All Journals  Testimonials  Membership  News  Donations


Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 32, No. 4, 2011, pp. 353-362
Bioline Code: zr11051
Full paper language: Chinese
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 32, No. 4, 2011, pp. 353-362

 en Analysis of the complete mitochondrial genome sequence of Pielomastax zhengi check for this species in other resources
Yang, Hui & Huang, Yuan

Abstract

The complete mitochondrial genome sequence of Pielomastax zhengi check for this species in other resources was determined by using long PCR and conserved primer walking approaches. The results showed that the entire mitochondrial genome of Pielomastax zhengi is 15 602 bp long with A+T content of 71.8%. All 37 genes are conserved in the positions observed in those of Locusta migratoria check for this species in other resources . All the genes are closely assembled by leaving 10 intergenic spacers in between. Those intergenic spacers are 47 bp in total (excluding the A+T rich region), with individual size ranges from 1 bp to 20 bp. In addition, there are a total of 52 bp overlapping sequences among 14 genes, ranging from 1−8 bp. All protein-coding genes start with a typical initiation codon in insects, ATN. Twelve protein-coding genes use the usual TAA and TAG termination codons, whereas, the ND5 genes have an incomplete termination codon (T). Except the tRNASer (AGN), whose DHU arm is absent; all the other 21 tRNA genes have typical clover-leaf secondary structures. But in Pielomastax zhengi, the secondary structures of five tRNA (tRNACys, tRNALys, tRNAPhe, tRNAPro, tRNAArg) genes can not be predicted by the conventional methods as they have only 3−4, rather than 5 base pairs in the TψC arm, while, the tRNALys and tRNAArg have only 4 base pairs in the anticode arm. The predicted secondary structures of lrRNA and srRNA have 6 domains with 44 helices and 3 domains with 30 helices, respectively. The results of the rRNA secondary structure comparison showed that Pielomastax zhengi is more closely related with Locusta migratoria tibetensis check for this species in other resources than Ruspolia dubia check for this species in other resources . Like most insects, the mitochondrial genome of Pielomastax zhengi has a non-coding A+T rich region containing a polythymidine stretch, which may be involved in the replication and/or translation initiation of other genes.

Keywords
Eumastacoidea; Pielomastax zhengi; Mitochondrial genome; RNA secondary structure

 
© Copyright 2011 Kunming Institute of Zoology, the Chinese Academy of Sciences
Alternative site location: http://www.zoores.ac.cn/

Home Faq Resources Email Bioline
© Bioline International, 1989 - 2017, Site last up-dated on 16-Oct-2017.
Site created and maintained by the Reference Center on Environmental Information, CRIA, Brazil
System hosted by the Internet Data Center of Rede Nacional de Ensino e Pesquisa, RNP, Brazil