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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 32, No. 4, 2011, pp. 461-464
Bioline Code: zr11065
Full paper language: Chinese
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 32, No. 4, 2011, pp. 461-464

 en Localization of stationary pronuclei during conjugation of Paramecium check for this species in other resources as indicated by immunofluorescence staining
Gao, Xin; Zhu, Jia-Jun; Yang, Xian-Yu; Yuan, Jin-Qiang; Wang, Yi-Wen & Song, Min-Guo

Abstract

After the third prezygotic division during conjugation of Paramecium caudatum check for this species in other resources , migratory and stationary pronuclei are produced. The migratory pronuclei remain in the paroral region tightly against the conjugating boundaries; while the stationary pronuclei are located beside the migratory pronuclei. To date, however, it is not clear what causes this close side-by-side localization between migratory and stationary pronuclei. In the current study, immunofluorescence staining with monoclonal antibody of anti-α tubulin indicated that "U" or "V" shaped spindles connected the migratory and stationary pronuclei during the third prezygotic division. This observation accounts for the close localization between these two types of pronuclei.

Keywords
Paramecium; Conjugation; Connecting spindles; Pronucleus

 
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