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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 32, No. 5, 2011, pp. 492-498
Bioline Code: zr11069
Full paper language: Chinese
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 32, No. 5, 2011, pp. 492-498

 en Alteration on the expression of ayu coagulation factor X gene upon Listonella anguillarum check for this species in other resources infection
HUANG, Zuo-An; CHEN, Jiong; LU, Xin-Jiang; SHI, Yu-Hong & LI, Ming-Yun

Abstract

Coagulation factor X (FX) plays an important role in the immune response of mammals. In this study, the full length cDNA sequence of the ayu FX gene, 1817 bp in length excluding 3'-polyA tail, was determined for the first time. The sequence contained an open reading frame, which encoded a protein of 453 amino acids with a molecular weight of 5.07 × 104. The predicted protein had motifs typical of animal FX, and its N-terminal 24 residues were the signal peptides. Sequence comparison showed that ayu FX shared 53% amino acid sequence identity with zebrafish FX. In healthy ayu, FX mRNA was expressed mainly in the liver and weakly in the brain and gill. After Listonella anguillarum check for this species in other resources infection, liver FX transcriptions significantly increased, and peaked at 16 h post infection. The serine protease motif of ayu FX was expressed in Escherichia coli check for this species in other resources and was subsequently used for antiserum preparation. Western blotting analysis revealed that serum FX significantly increased in bacterially infected ayu fish. In conclusion, the ayu FX gene expression was significant in the progress of bacterial infection, which suggests FX’s role in fish immune response.

Keywords
Coagulation factor FX, Ayu, Listonella anguillarum, RT-qPCR, Western blotting

 
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