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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 32, No. 6, 2011, pp. 641-646
Bioline Code: zr11092
Full paper language: Chinese
Document type: Report
Document available free of charge

Zoological Research, Vol. 32, No. 6, 2011, pp. 641-646

 en Cloning of hemoglobin-α1 from half-smooth tongue sole ( Cynoglossus semilaevis check for this species in other resources ) and its expression under short-term hypoxia
WANG, Zi-Sheng; QI, Zhi-Tao; TIAN, Jing-Yun; QIU, Ming; ZHAO, Wei-Hong; WANG, Ai-Min; HUANG, Jin-Tian & GUO, Xi-Jie

Abstract

This study cloned the hemoglobin α1 from the marine teleost, the half-smooth tongue sole ( Cynoglossus semilaevis check for this species in other resources ), and then examined its expression under hypoxia exposure. The full-length of CsHb-α1 (594 bp) cDNA contains an open reading frame encoding 144 amino acids. Sequence analysis shows that the predicted CsHb-α1 amino acids shares high identities with that of other species. Real-time PCR showed that CsHb-α1 was highly expressed in the heart, liver, spleen, kidney and blood. Five to 120 min esposure and long-term (36 h) exposure to hypoxia (1.0 mg/L) significantly increased CsHb-α1 mRNA expression in most tissues compared to those fish held in normoxic conditions (dissolved oxygen (DO): 6.2 mg/L). These results suggested that the up-regulation of Hb-α1 is an important component for adaptation of half-smooth tongue sole to short-term hypoxia.

Keywords
Cynoglossus semilaevis, Hemoglobin, Gene clone, Hypoxia stress

 
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