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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 32, No. 6, 2011, pp. 651-656
Bioline Code: zr11094
Full paper language: Chinese
Document type: Report
Document available free of charge

Zoological Research, Vol. 32, No. 6, 2011, pp. 651-656

 en New details indicated by different stainings during conjugation of ciliated protozoa Paramecium check for this species in other resources
GAO, Xin; YANG, Xian-Yu; ZHU, Jia-Jun; YUAN, Jin-Qiang; WANG, Yi-Wen & SONG, Min-Guo


During conjugation of Paramecium c audatum check for this species in other resources , nuclear events occur in a scheduled program. Morphological studies on nuclear behavior during conjugation of P. caudatum have been performed since the end of the 19th century. Here we report on new details concerning the conjugation of P. caudatum through the staining of conjugating cells with protargol, carbol fuchsin solution, Hoechst 33342 and immunofluorescence labeling with monoclonal antibody of anti-α tubulin. 1) The crescent nucleus is a characteristic of the meiotic prophase of P. caudatum, has an unstained area. We stained this area with protargol, which was separated from the chromatin area and was not detected by the other stainings. 2) In regards to the four meiotic products, it has long been considered that only one product enters the paroral cone region (PC) and survives after meiosis. However, our protargol and immunofluorescence labeling results indicated that PC entrance of the meiotic product happened before the completion of meiosis instead of after. 3) In our previous study, protargol staining indicated the presence of a swollen structure around the central part of the "U" and "V" shaped spindles connecting the two types of prospective pronuclei. However, immunofluorescence labeling with anti-α tubulin antibodies gave a different image from protargol. All these observations form the basis for further studies of their molecular mechanisms.

Paramecium, Conjugation, Crescent, PC entrance, Connecting spindles

© Copyright 2011 Kunming Institute of Zoology, the Chinese Academy of Sciences
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