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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 33, No. 1, 2012, pp. 1-6
Bioline Code: zr12001
Full paper language: English
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 33, No. 1, 2012, pp. 1-6

 en Two bacterial infection models in tree shrew for evaluating the efficacy of antimicrobial agents
LI, Sheng-An; LEE, Wen-Hui & ZHANG, Yun

Abstract

Animal models are essential for the development of new anti-infectious drugs. Although some bacterial infection models have been established in rodents, small primate models are rare. Here, we report on two bacterial infection models established in tree shrew ( Tupaia belangeri chinensis check for this species in other resources ). A burnt skin infection model was induced by dropping 5×106 CFU of Staphylococcus aureus check for this species in other resources on the surface of a wound after a third degree burn. This dose of S. aureus caused persistent infection for 7 days and obvious inflammatory response was observed 4 days after inoculation. A Dacron graft infection model, 2×106 CFU of Pseudomonas aeruginosa check for this species in other resources also caused persistent infection for 6 days, with large amounts of pus observed 3 days after inoculation. These models were used to evaluate the efficacy of levofloxacin (LEV) and cefoperazone (CPZ), which reduced the viable bacteria in skin to 4log10 and 5log10 CFU/100 mg tissue, respectively. The number of bacteria in graft was significantly reduced by 4log10 CFU/mL treatment compared to the untreated group (P<0.05). These results suggest that two bacterial infection models were successfully established in tree shrew using P. aeruginosa and S. aureus. In addition, tree shrew was susceptible to P. aeruginosa and S. aureus, thus making it an ideal bacterial infection animal model for the evaluation of new antimicrobials.

Keywords
Tree shrew; Novel antimicrobials; Graft infection; Staphylococcus aureus; Pseudomonas aeruginosa

 
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