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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 33, No. 1, 2012, pp. 43-48
Bioline Code: zr12007
Full paper language: English
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 33, No. 1, 2012, pp. 43-48

 en Rhesus monkey embryonic stem cells differentiation, proliferation and allotransplantation
DONG, Jin-Run; GUO, Li-Yun; QU, Jia-Gui; QI, Ren-Li; WANG, Wen-Chao; XIAO, Chun-Jie & WANG, Zheng-Bo

Abstract

To investigate the characteristics of rhesus monkey embryonic stem cells and to promote their clinical application, the differentiation and proliferation of rosettes neural stem cells from GFP marked rhesus monkey embryonic stem cells were studied The results showed that: 1) A stable and high-efficient neural differentiation system was established. More than 95% of the embryonic stem cells were differentiated into neural stem cells on the 12th days of differentiation; 2) the rosettes neural stem cells differentiated from the rhesus monkey embryonic stem cells could maintain their rosettes-shape by proliferating with bFGF/EGF; 3) the neural stem cells could differentiate into neurons after transplanted into the rhesus monkey brain. In conclusion, the rosettes neural stem cells differentiated from rhesus monkey embryonic stem cells could maintain their characteristics after proliferation with bFGF/EGF and they could survive and differentiate into neurons after transplanted into the rhesus monkey brain, which strongly supports the clinical application of neural stem cells in the future.

Keywords
Rhesus monkey; Neural stem cells; Rosettes; Basic fibroblast growth factor; Epidermal growth factor

 
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