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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 33, No. 1, 2012, pp. 60-66
Bioline Code: zr12010
Full paper language: English
Document type: Report
Document available free of charge

Zoological Research, Vol. 33, No. 1, 2012, pp. 60-66

 en Cloning of full-length coding sequence of tree shrew CD4 and prediction of its molecular characteristics
TIAN, Wei-Wei; GAO, Yue-Dong; GUO, Yan; HUANG, Jing-Fei; XIAO, Chang; LI, Zuo-Sheng & ZHANG, Hua-Tang

Abstract

The tree shrews, as an ideal animal model receiving extensive attentions to human disease research, demands essential research tools, in particular cellular markers and monoclonal antibodies for immunological studies. In this paper, a 1 365 bp of the full-length CD4 cDNA encoding sequence was cloned from total RNA in peripheral blood of tree shrews, the sequence completes two unknown fragment gaps of tree shrews predicted CD4 cDNA in the GenBank database, and its molecular characteristics were analyzed compared with other mammals by using biology software such as Clustal W2.0 and so forth. The results showed that the extracellular and intracellular domains of tree shrews CD4 amino acid sequence are conserved. The tree shrews CD4 amino acid sequence showed a close genetic relationship with Homo sapiens check for this species in other resources and Macaca mulatta check for this species in other resources . Most regions of the tree shrews CD4 molecule surface showed positive charges as humans. However, compared with CD4 extracellular domain D1 of human, CD4 D1 surface of tree shrews showed more negative charges, and more two N-glycosylation sites, which may affect antibody binding. This study provides a theoretical basis for the preparation and functional studies of CD4 monoclonal antibody.

Keywords
Tree shrews; CD4; RT-PCR; Structure; Function; Monoclonal antibody

 
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