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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 33, No. 2, 2012, pp. 203-210
Bioline Code: zr12031
Full paper language: English
Document type: Report
Document available free of charge

Zoological Research, Vol. 33, No. 2, 2012, pp. 203-210

 en Analysis of genetic diversity in wild populations of Trachidermus fasciatus check for this species in other resources by RAPD and the transformation of two SCAR markers
ZENG, Zhen; LIU, Zhi-Zhi; PAN, Lian-De; TANG, Wen-Qiao; WANG, Qian & GENG, Yun-Hao

Abstract

Firstly, RAPD was conducted to analyze genetic diversity of Trachidermus fasciatus check for this species in other resources in the Fuchun River population (FR), Yellow River population (YR), Luan River population (LR), and Yalu River population (YL), with 32 polymorphic 10-bp random primers selected from 294 ones. Thirty wild individuals were detected in each population. The results indicated that the genetic diversity of T . fasciatus was relatively rich. The major results were as the following: 1) Altogether, 591 bands were detected and 515 of them were polymorphic, accounted for 87.14%. The range of proportion of polymorphic loci (P) was: FR(89.17%)>YR(87.99%)>YL(86.63%)>LR(83.25%). 2) The Shannon’s information index(IT) and Nei’s genetic diversity(HT) among populations were 0.3393−0.3566 and 0.2157−0.2279, respectively. Compare to other three populations, LR population had relative lower values. If took the populations as a whole, the total Nei’s genetic diversity(HT) and Shannon’s information index(IT) was 0.2336±0.1643 and 0.3710±0.2153, respectively. 3) The value of gene flow (Nm) (5.76103−19.84497) were high, indicating certain gene exchange existed among the four populations. But the AMOVA results exhibited significantly differentiation (P<0.05 or P<0.01) among the populations. 4) In the UPGMA tree constructed according to genetic distance, YL and YR populations clustered firstly, then with FR population, and finally they joined to LR population. Obviously, the YL, YR and FR populations had relatively close relationship according to their geographic distance, whereas LR population showed clear divergence to the other three populations. Secondly, out of the five special RAPD bands (S1225 525 bp, S1225 605 bp, S1225 841 bp, S1345 695 bp and S1345 825 bp), SCAR maker SCAR01560 bp and SCAR02443 bp were successfully transformed from S1255 605 bp and S1255 841 bp, respectively. After large samples examination of the two markers, we found the highest frequency (96.67% and 93.33%) in the YL population, higher frequency (83.33% and 90%) in the FR population, high frequency (56.67% and 66.67%) in the YR population, and the lowest frequency (13.33% and 20 %) in the LR population. Therefore, SCAR01560 bp and SCAR02443 bp can be used as special molecular markers for the population identification between LR and other three populations.

Keywords
Trachidermus fasciatus; Genetic diversity; RAPD; SCAR marker

 
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