Pseudosciaena polyactis
is an economically important species of marine fish in China that is currently
declining due to overexploitation, environmental pollution and related factors. Research in to the genetic structure of
Pseudosciaena polyactis populations plays a key role in protecting and promoting sustainable utilization. We collected 53
individuals of
Pseudosciaena polyactis from Zhoushan, Zhejiang and sequenced and amplified the mitochondrial DNA
(mtDNA) D-loop region using Polymerase Chain Reactions (PCR). The sequence length of the 53 individuals ranged
from 795 to 801 bp. The sequences were analyzed by Clustal X1.83, MEGA3.1 and DnaSP4.0. The results showed that
the average base content of T, C, A, G was 30.3%, 23.1%, 32.3% and 14.3%, respectively and there were 93 transition or
transversion sites, including 53 single nucleotide mutation sites and 40 parsimony informative sites, which accounted for
11.6% of the length of the analyzed sequences. In total, we identified 52 haplotypes and found haplotype diversity (
hd) of
0.9993, average number of nucleotide differences were 9.73875 (
k), and nucleotide diversity (
π) of 0.01233. The average
genetic distance of haplotypes was 0.012, and the average transition/transversion was 4.305. Based on mitochondrial
DNA D-loop region sequences, these results indicate that the genetic diversity of the
Pseudosciaena polyactis population
in Zhoushan is currently at a medium level.