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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 33, No. 3, 2012, pp. 329-336
Bioline Code: zr12049
Full paper language: English
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 33, No. 3, 2012, pp. 329-336

 en Genetic diversity of Pseudosciaena polyactis check for this species in other resources in Zhoushan based on mitochondrial DNA D-loop region sequences
ZHENG, Wen-Juan; LAI, Yu-Hong; YOU, Xin-Yu; QIN, Xi-Han & ZHU, Shi-Hua

Abstract

Pseudosciaena polyactis check for this species in other resources is an economically important species of marine fish in China that is currently declining due to overexploitation, environmental pollution and related factors. Research in to the genetic structure of Pseudosciaena polyactis populations plays a key role in protecting and promoting sustainable utilization. We collected 53 individuals of Pseudosciaena polyactis from Zhoushan, Zhejiang and sequenced and amplified the mitochondrial DNA (mtDNA) D-loop region using Polymerase Chain Reactions (PCR). The sequence length of the 53 individuals ranged from 795 to 801 bp. The sequences were analyzed by Clustal X1.83, MEGA3.1 and DnaSP4.0. The results showed that the average base content of T, C, A, G was 30.3%, 23.1%, 32.3% and 14.3%, respectively and there were 93 transition or transversion sites, including 53 single nucleotide mutation sites and 40 parsimony informative sites, which accounted for 11.6% of the length of the analyzed sequences. In total, we identified 52 haplotypes and found haplotype diversity (hd) of 0.9993, average number of nucleotide differences were 9.73875 (k), and nucleotide diversity (π) of 0.01233. The average genetic distance of haplotypes was 0.012, and the average transition/transversion was 4.305. Based on mitochondrial DNA D-loop region sequences, these results indicate that the genetic diversity of the Pseudosciaena polyactis population in Zhoushan is currently at a medium level.

Keywords
Mitochondrial DNA; D-loop region; Pseudosciaena polyactis; Genetic diversity; Zhoushan

 
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Alternative site location: http://www.zoores.ac.cn/

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