search
for
 About Bioline  All Journals  Testimonials  Membership  News  Donations


Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 33, No. 5, 2012, pp. 487-492
Bioline Code: zr12076
Full paper language: English
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 33, No. 5, 2012, pp. 487-492

 en Analysis of differentially expressed genes from female and male postparasitic Ovomermis sinensis check for this species in other resources juveniles
WU, Yun-Mei; ZHANG, Li-Hong & WANG, Guo-Xiu

Abstract

Mermithidae, as an important natural predator of pests such as Bollworm, has great potential for natural biological control of invasive pests. Unfortunately, the in vitro culture of the nematode has not yet been successful, delaying the commercial application of this pest control method. The key reason for this failure is the inability of the worms to accomplish sex differentiation, sparking a strong interest in this process. Here, we analyzed the differences in gene expression of female and male postparasitic Ovomermis sinensis check for this species in other resources juveniles by mRNA differential display. In total, 20 gene fragments that had differential expression in male and female worms were isolated, including 8 male- and 12 female-specific ones. Bioinformatics methods were employed to analyze sequences of these fragments, in which ensembl analysis shows 4 fragments have comparable parts with C. elegence’s check for this species in other resources X chromosome, we speculate those fragments are important genes which influence sex differentiation of Ovomermis sinensis, This data provides an idea for further study of the molecular mechanism of sex differentiation in mermithids.

Keywords
Mermithidae; Sexual differentiation; mRNA differentially display

 
© Copyright 2012 - Kunming Institute of Zoology, the Chinese Academy of Sciences
Alternative site location: http://www.zoores.ac.cn/

Home Faq Resources Email Bioline
© Bioline International, 1989 - 2017, Site last up-dated on 05-Dec-2017.
Site created and maintained by the Reference Center on Environmental Information, CRIA, Brazil
System hosted by the Internet Data Center of Rede Nacional de Ensino e Pesquisa, RNP, Brazil