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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 34, No. 2, 2013, pp. 62-68
Bioline Code: zr13019
Full paper language: English
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 34, No. 2, 2013, pp. 62-68

 en Characterization of 12 polymorphic microsatellite markers in the Chinese tree shrew ( Tupaia belangeri chinensis check for this species in other resources )
LIU, Xiao-Hong & YAO, Yong-Gang

Abstract

The Chinese tree shrew ( Tupaia belangeri chinensis check for this species in other resources ) is a small experimental animal with a close affinity to primates. This species has long been proposed to be an alternative experimental animal to primates in biomedical research. Despite decades of study, there is no pure breed for this animal, and the overall genetic diversity of wild tree shrews remains largely unknown. In order to obtain a set of genetic markers for evaluating the genetic diversity of tree shrew wild populations and tracing the lineages in inbreeding populations, we developed 12 polymorphic microsatellite markers from the genomic DNA of the tree shrew. An analysis of a wild population of 117 individuals collected from the suburb of Kunming, China, showed that these loci exhibited a highly expected heterozygosity (0.616). These 12 microsatellites were sufficient for individual identification and parentage analysis. The microsatellite markers developed in this study will be of use in evaluating genetic diversity and lineage tracing for the tree shrew.

Keywords
Chinese tree shrew; Microsatellite; Heterozygosity; Individual identification; Parentage testing

 
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