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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 34, No. 2, 2013, pp. 121-126
Bioline Code: zr13028
Full paper language: English
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 34, No. 2, 2013, pp. 121-126

 en Analysis of the molecular characteristics and cloning of full-length coding sequence of Interleukin-2 in tree shrews
HUANG, Xiao-Yan; LI, Ming-Li; XU, Juan; GAO, Yue-Dong; WANG, Wen-Guang; YIN, An-Guo; LI, Xiao-Fei; SUN, Xiao-Mei; XIA, Xue-Shan & DAI, Jie-Jie

Abstract

While the tree shrew ( Tupaia belangeri chinensis check for this species in other resources ) is an excellent animal model for studying the mechanisms of human diseases, but few studies examine interleukin-2 (IL-2), an important immune factor in disease model evaluation. In this study, a 465 bp of the full-length IL-2 cDNA encoding sequence was cloned from the RNA of tree shrew spleen lymphocytes, which were then cultivated and stimulated with ConA (concanavalin). Clustal W 2.0 was used to compare and analyze the sequence and molecular characteristics, and establish the similarity of the overall structure of IL-2 between tree shrews and other mammals. The homology of the IL-2 nucleotide sequence between tree shrews and humans was 93%, and the amino acid homology was 80%. The phylogenetic tree results, derived through the Neighbour-Joining method using MEGA5.0, indicated a close genetic relationship between tree shrews, Homo sapiens check for this species in other resources , and Macaca mulatta check for this species in other resources . The three-dimensional structure analysis showed that the surface charges in most regions of tree shrew IL-2 were similar to between tree shrews and humans; however, the N-glycosylation sites and local structures were different, which may affect antibody binding. These results provide a fundamental basis for the future study of IL-2 monoclonal antibody in tree shrews, thereby improving their utility as a model.

Keywords
Tree shrews; Interleukin 2; Cloning; Structure; Function

 
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