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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 34, No. 3, 2013, pp. 87-92
Bioline Code: zr13033
Full paper language: English
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 34, No. 3, 2013, pp. 87-92

 en Pilot study on binding of bovine salivary proteins to grit silicates and plant phytoliths
MAU, Marcus; KAISER, Thomas M. & SÜDEKUM, Karl-Heinz

Abstract

Mostly fed with grass in fresh or conserved form, cattle and other livestock have to cope with silicate defence bodies from plants (phytoliths) and environmental silicates (grit), which abrade tooth enamel and could additionally interact with various salivary proteins. To detect potential candidates for silicate-binding proteins, bovine whole saliva was incubated with grass-derived phytoliths and silicates. Interactions of salivary proteins with pulverized bovine dental enamel and dentine were additionally analysed. After intense washing, the powder fractions were loaded onto 1D-polyacrylamide gels, most prominent adhesive protein bands were cut out and proteins were identified by mass spectrometry within three independent replicates. All materials were mainly bound by bovine odorant-binding protein, bovine salivary protein 30×103 and carbonic anhydrase VI. The phytolith/silicate fraction showed additional stronger interaction with haemoglobin β and lactoperoxidase. Conceivably, the binding of these proteins to the surfaces may contribute to biological processes occurring on them.

Keywords
Bovine salivary proteins; Mass spectrometry; Phytoliths; Silicates; Tooth enamel

 
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