The physiological and behavioral differences between
Myotis lucifugus
and
Pteropus vampyrus
should be attributed to molecular mechanisms and deserve intensive investigation. We conducted genome-wide scan for coding sequences from the
orthologue genes of seven mammalian species. Selection analyses were carried out by setting the branches leading to
Myotis
lucifugus and
Pteropus vampyrus as foreground branches, respectively. Enrichment analyses were conducted for positively selected
genes. Our results indicated that more genes exhibited positive selection in
Myotis lucifugus than that in
Pteropus vampyrus. The
positively selected genes of the two species were enriched in different functions. The differences between
Myotis lucifugus and
Pteropus vampyrus represented their differentiation in biological functions, especially the functions of immunity, motor ability,
energetic metabolism and sensory organ development.