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Diabetes induced by multiple low doses of STZ can be spontaneously recovered in adult mice
LI, Ru-Jiang; QIU, Shu-Dong; TIAN, Hong & ZHOU, Sheng-Wei
Abstract
In a previous study, we observed that multiple low doses of streptozotocin (STZ) can induce spontaneous restoration of
normoglycemia in adult mouse diabetes models. In the present study, we sought to identify when spontaneous recovery from diabetes
occurs and to disclose the changes in the diabetic data of diabetic mice induced by multiple low doses of streptozotocin (MLD-STZ
mice). After inducing type 1 diabetes mellitus, radioimmunoassay, indirect immunofluorescence and hematoxylin-eosin staining were
used to determine the diabetic data of MLD-STZ mice. In MLD-STZ mice, the diabetic indicators, including food intake, water
consumption, body weight, blood glucose level and serum insulin concentration, developed gradually until week 20. Thereafter, the
symptoms of diabetes gradually improved. By the week 36, although body weight and β cell masses remained significantly different
between the MLD-STZ mice and the age-matched control animals, food intake, water consumption, blood glucose levels and serum
insulin concentrations had all returned to normal levels, and no lymphocytic infiltrations were observed in pancreatic islets. This data
demonstrates that MLD-STZ can induce spontaneous recovery from diabetes mellitus in adult mice, suggesting further research into
the processes by which normoglycemia is recovered.
Keywords
Streptozotocin; Type 1 diabetes mellitus; Blood glucose; β cell mass; Mouse
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